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( A ) Top, schematic of retrograde AAV <t>(rAAV-hSyn-EGFP)</t> injection into ventral retrohippocampal areas of a wild-type mouse. PaS, parasubiculum. Bottom, widefield epifluorescence of a coronal brain section (case SH63) containing retrogradely labeled cells in <t>the</t> <t>ADn</t> (dashed area) expressing EGFP (green). Note descending axons originating from the medial ADn (e.g. white arrows). Scale bar, 100 µm. ( B ) Retrogradely labeled cells (EGFP+, green) were detected in the ADn and had a similar distribution to CR (red). Case SH63, confocal maximum intensity z-projection (9 µm thick). Scale bar, 100 µm. ( C ) Intensity profiles for GFP and CR. ( D ) Pie chart illustrating the proportion of CR+ cells within the total population of GFP+ cells. ( E ) Pie chart illustrating the proportion of C1ql2+ cells within the total population of CR+ cells. ( F ) Top, schematic of a Cre-dependent retrograde AAV (rAAV-hSyn-DIO-EGFP) injection into the dorso-caudal region of the retrosplenial cortex (RSp) and presubiculum (PrS) of a C1ql2-Cre mouse. Bottom, widefield epifluorescence of a coronal brain section showing selective labeling in the ADn (dashed area), case SH85. Scale bar, 100 µm. ( G ) Retrogradely labeled cells (EGFP+, green) were detected in the ADn and had very little colocalization with CR (red). Note axons originating from the ADn heading laterally towards the TRN (e.g. white arrows). Case SH63, confocal maximum intensity z-projection (9 µm thick). Scale bar, 100 µm. ( H ) Intensity profiles for GFP and CR. ( I ) Pie chart illustrating the proportion of CR+ cells within the total population of GFP+ cells.
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( A ) Top, schematic of retrograde AAV <t>(rAAV-hSyn-EGFP)</t> injection into ventral retrohippocampal areas of a wild-type mouse. PaS, parasubiculum. Bottom, widefield epifluorescence of a coronal brain section (case SH63) containing retrogradely labeled cells in <t>the</t> <t>ADn</t> (dashed area) expressing EGFP (green). Note descending axons originating from the medial ADn (e.g. white arrows). Scale bar, 100 µm. ( B ) Retrogradely labeled cells (EGFP+, green) were detected in the ADn and had a similar distribution to CR (red). Case SH63, confocal maximum intensity z-projection (9 µm thick). Scale bar, 100 µm. ( C ) Intensity profiles for GFP and CR. ( D ) Pie chart illustrating the proportion of CR+ cells within the total population of GFP+ cells. ( E ) Pie chart illustrating the proportion of C1ql2+ cells within the total population of CR+ cells. ( F ) Top, schematic of a Cre-dependent retrograde AAV (rAAV-hSyn-DIO-EGFP) injection into the dorso-caudal region of the retrosplenial cortex (RSp) and presubiculum (PrS) of a C1ql2-Cre mouse. Bottom, widefield epifluorescence of a coronal brain section showing selective labeling in the ADn (dashed area), case SH85. Scale bar, 100 µm. ( G ) Retrogradely labeled cells (EGFP+, green) were detected in the ADn and had very little colocalization with CR (red). Note axons originating from the ADn heading laterally towards the TRN (e.g. white arrows). Case SH63, confocal maximum intensity z-projection (9 µm thick). Scale bar, 100 µm. ( H ) Intensity profiles for GFP and CR. ( I ) Pie chart illustrating the proportion of CR+ cells within the total population of GFP+ cells.
Raav Hsyn Con Fon Hchr2 H134r Eyfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) Top, schematic of retrograde AAV <t>(rAAV-hSyn-EGFP)</t> injection into ventral retrohippocampal areas of a wild-type mouse. PaS, parasubiculum. Bottom, widefield epifluorescence of a coronal brain section (case SH63) containing retrogradely labeled cells in <t>the</t> <t>ADn</t> (dashed area) expressing EGFP (green). Note descending axons originating from the medial ADn (e.g. white arrows). Scale bar, 100 µm. ( B ) Retrogradely labeled cells (EGFP+, green) were detected in the ADn and had a similar distribution to CR (red). Case SH63, confocal maximum intensity z-projection (9 µm thick). Scale bar, 100 µm. ( C ) Intensity profiles for GFP and CR. ( D ) Pie chart illustrating the proportion of CR+ cells within the total population of GFP+ cells. ( E ) Pie chart illustrating the proportion of C1ql2+ cells within the total population of CR+ cells. ( F ) Top, schematic of a Cre-dependent retrograde AAV (rAAV-hSyn-DIO-EGFP) injection into the dorso-caudal region of the retrosplenial cortex (RSp) and presubiculum (PrS) of a C1ql2-Cre mouse. Bottom, widefield epifluorescence of a coronal brain section showing selective labeling in the ADn (dashed area), case SH85. Scale bar, 100 µm. ( G ) Retrogradely labeled cells (EGFP+, green) were detected in the ADn and had very little colocalization with CR (red). Note axons originating from the ADn heading laterally towards the TRN (e.g. white arrows). Case SH63, confocal maximum intensity z-projection (9 µm thick). Scale bar, 100 µm. ( H ) Intensity profiles for GFP and CR. ( I ) Pie chart illustrating the proportion of CR+ cells within the total population of GFP+ cells.
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( A ) Top, schematic of retrograde AAV <t>(rAAV-hSyn-EGFP)</t> injection into ventral retrohippocampal areas of a wild-type mouse. PaS, parasubiculum. Bottom, widefield epifluorescence of a coronal brain section (case SH63) containing retrogradely labeled cells in <t>the</t> <t>ADn</t> (dashed area) expressing EGFP (green). Note descending axons originating from the medial ADn (e.g. white arrows). Scale bar, 100 µm. ( B ) Retrogradely labeled cells (EGFP+, green) were detected in the ADn and had a similar distribution to CR (red). Case SH63, confocal maximum intensity z-projection (9 µm thick). Scale bar, 100 µm. ( C ) Intensity profiles for GFP and CR. ( D ) Pie chart illustrating the proportion of CR+ cells within the total population of GFP+ cells. ( E ) Pie chart illustrating the proportion of C1ql2+ cells within the total population of CR+ cells. ( F ) Top, schematic of a Cre-dependent retrograde AAV (rAAV-hSyn-DIO-EGFP) injection into the dorso-caudal region of the retrosplenial cortex (RSp) and presubiculum (PrS) of a C1ql2-Cre mouse. Bottom, widefield epifluorescence of a coronal brain section showing selective labeling in the ADn (dashed area), case SH85. Scale bar, 100 µm. ( G ) Retrogradely labeled cells (EGFP+, green) were detected in the ADn and had very little colocalization with CR (red). Note axons originating from the ADn heading laterally towards the TRN (e.g. white arrows). Case SH63, confocal maximum intensity z-projection (9 µm thick). Scale bar, 100 µm. ( H ) Intensity profiles for GFP and CR. ( I ) Pie chart illustrating the proportion of CR+ cells within the total population of GFP+ cells.
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( A ) Top, schematic of retrograde AAV <t>(rAAV-hSyn-EGFP)</t> injection into ventral retrohippocampal areas of a wild-type mouse. PaS, parasubiculum. Bottom, widefield epifluorescence of a coronal brain section (case SH63) containing retrogradely labeled cells in <t>the</t> <t>ADn</t> (dashed area) expressing EGFP (green). Note descending axons originating from the medial ADn (e.g. white arrows). Scale bar, 100 µm. ( B ) Retrogradely labeled cells (EGFP+, green) were detected in the ADn and had a similar distribution to CR (red). Case SH63, confocal maximum intensity z-projection (9 µm thick). Scale bar, 100 µm. ( C ) Intensity profiles for GFP and CR. ( D ) Pie chart illustrating the proportion of CR+ cells within the total population of GFP+ cells. ( E ) Pie chart illustrating the proportion of C1ql2+ cells within the total population of CR+ cells. ( F ) Top, schematic of a Cre-dependent retrograde AAV (rAAV-hSyn-DIO-EGFP) injection into the dorso-caudal region of the retrosplenial cortex (RSp) and presubiculum (PrS) of a C1ql2-Cre mouse. Bottom, widefield epifluorescence of a coronal brain section showing selective labeling in the ADn (dashed area), case SH85. Scale bar, 100 µm. ( G ) Retrogradely labeled cells (EGFP+, green) were detected in the ADn and had very little colocalization with CR (red). Note axons originating from the ADn heading laterally towards the TRN (e.g. white arrows). Case SH63, confocal maximum intensity z-projection (9 µm thick). Scale bar, 100 µm. ( H ) Intensity profiles for GFP and CR. ( I ) Pie chart illustrating the proportion of CR+ cells within the total population of GFP+ cells.
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( A ) Top, schematic of retrograde AAV <t>(rAAV-hSyn-EGFP)</t> injection into ventral retrohippocampal areas of a wild-type mouse. PaS, parasubiculum. Bottom, widefield epifluorescence of a coronal brain section (case SH63) containing retrogradely labeled cells in <t>the</t> <t>ADn</t> (dashed area) expressing EGFP (green). Note descending axons originating from the medial ADn (e.g. white arrows). Scale bar, 100 µm. ( B ) Retrogradely labeled cells (EGFP+, green) were detected in the ADn and had a similar distribution to CR (red). Case SH63, confocal maximum intensity z-projection (9 µm thick). Scale bar, 100 µm. ( C ) Intensity profiles for GFP and CR. ( D ) Pie chart illustrating the proportion of CR+ cells within the total population of GFP+ cells. ( E ) Pie chart illustrating the proportion of C1ql2+ cells within the total population of CR+ cells. ( F ) Top, schematic of a Cre-dependent retrograde AAV (rAAV-hSyn-DIO-EGFP) injection into the dorso-caudal region of the retrosplenial cortex (RSp) and presubiculum (PrS) of a C1ql2-Cre mouse. Bottom, widefield epifluorescence of a coronal brain section showing selective labeling in the ADn (dashed area), case SH85. Scale bar, 100 µm. ( G ) Retrogradely labeled cells (EGFP+, green) were detected in the ADn and had very little colocalization with CR (red). Note axons originating from the ADn heading laterally towards the TRN (e.g. white arrows). Case SH63, confocal maximum intensity z-projection (9 µm thick). Scale bar, 100 µm. ( H ) Intensity profiles for GFP and CR. ( I ) Pie chart illustrating the proportion of CR+ cells within the total population of GFP+ cells.
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( A ) Top, schematic of retrograde AAV <t>(rAAV-hSyn-EGFP)</t> injection into ventral retrohippocampal areas of a wild-type mouse. PaS, parasubiculum. Bottom, widefield epifluorescence of a coronal brain section (case SH63) containing retrogradely labeled cells in <t>the</t> <t>ADn</t> (dashed area) expressing EGFP (green). Note descending axons originating from the medial ADn (e.g. white arrows). Scale bar, 100 µm. ( B ) Retrogradely labeled cells (EGFP+, green) were detected in the ADn and had a similar distribution to CR (red). Case SH63, confocal maximum intensity z-projection (9 µm thick). Scale bar, 100 µm. ( C ) Intensity profiles for GFP and CR. ( D ) Pie chart illustrating the proportion of CR+ cells within the total population of GFP+ cells. ( E ) Pie chart illustrating the proportion of C1ql2+ cells within the total population of CR+ cells. ( F ) Top, schematic of a Cre-dependent retrograde AAV (rAAV-hSyn-DIO-EGFP) injection into the dorso-caudal region of the retrosplenial cortex (RSp) and presubiculum (PrS) of a C1ql2-Cre mouse. Bottom, widefield epifluorescence of a coronal brain section showing selective labeling in the ADn (dashed area), case SH85. Scale bar, 100 µm. ( G ) Retrogradely labeled cells (EGFP+, green) were detected in the ADn and had very little colocalization with CR (red). Note axons originating from the ADn heading laterally towards the TRN (e.g. white arrows). Case SH63, confocal maximum intensity z-projection (9 µm thick). Scale bar, 100 µm. ( H ) Intensity profiles for GFP and CR. ( I ) Pie chart illustrating the proportion of CR+ cells within the total population of GFP+ cells.
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Image Search Results


( A ) Top, schematic of retrograde AAV (rAAV-hSyn-EGFP) injection into ventral retrohippocampal areas of a wild-type mouse. PaS, parasubiculum. Bottom, widefield epifluorescence of a coronal brain section (case SH63) containing retrogradely labeled cells in the ADn (dashed area) expressing EGFP (green). Note descending axons originating from the medial ADn (e.g. white arrows). Scale bar, 100 µm. ( B ) Retrogradely labeled cells (EGFP+, green) were detected in the ADn and had a similar distribution to CR (red). Case SH63, confocal maximum intensity z-projection (9 µm thick). Scale bar, 100 µm. ( C ) Intensity profiles for GFP and CR. ( D ) Pie chart illustrating the proportion of CR+ cells within the total population of GFP+ cells. ( E ) Pie chart illustrating the proportion of C1ql2+ cells within the total population of CR+ cells. ( F ) Top, schematic of a Cre-dependent retrograde AAV (rAAV-hSyn-DIO-EGFP) injection into the dorso-caudal region of the retrosplenial cortex (RSp) and presubiculum (PrS) of a C1ql2-Cre mouse. Bottom, widefield epifluorescence of a coronal brain section showing selective labeling in the ADn (dashed area), case SH85. Scale bar, 100 µm. ( G ) Retrogradely labeled cells (EGFP+, green) were detected in the ADn and had very little colocalization with CR (red). Note axons originating from the ADn heading laterally towards the TRN (e.g. white arrows). Case SH63, confocal maximum intensity z-projection (9 µm thick). Scale bar, 100 µm. ( H ) Intensity profiles for GFP and CR. ( I ) Pie chart illustrating the proportion of CR+ cells within the total population of GFP+ cells.

Journal: bioRxiv

Article Title: Granularity of thalamic head direction cells

doi: 10.1101/2025.09.08.674912

Figure Lengend Snippet: ( A ) Top, schematic of retrograde AAV (rAAV-hSyn-EGFP) injection into ventral retrohippocampal areas of a wild-type mouse. PaS, parasubiculum. Bottom, widefield epifluorescence of a coronal brain section (case SH63) containing retrogradely labeled cells in the ADn (dashed area) expressing EGFP (green). Note descending axons originating from the medial ADn (e.g. white arrows). Scale bar, 100 µm. ( B ) Retrogradely labeled cells (EGFP+, green) were detected in the ADn and had a similar distribution to CR (red). Case SH63, confocal maximum intensity z-projection (9 µm thick). Scale bar, 100 µm. ( C ) Intensity profiles for GFP and CR. ( D ) Pie chart illustrating the proportion of CR+ cells within the total population of GFP+ cells. ( E ) Pie chart illustrating the proportion of C1ql2+ cells within the total population of CR+ cells. ( F ) Top, schematic of a Cre-dependent retrograde AAV (rAAV-hSyn-DIO-EGFP) injection into the dorso-caudal region of the retrosplenial cortex (RSp) and presubiculum (PrS) of a C1ql2-Cre mouse. Bottom, widefield epifluorescence of a coronal brain section showing selective labeling in the ADn (dashed area), case SH85. Scale bar, 100 µm. ( G ) Retrogradely labeled cells (EGFP+, green) were detected in the ADn and had very little colocalization with CR (red). Note axons originating from the ADn heading laterally towards the TRN (e.g. white arrows). Case SH63, confocal maximum intensity z-projection (9 µm thick). Scale bar, 100 µm. ( H ) Intensity profiles for GFP and CR. ( I ) Pie chart illustrating the proportion of CR+ cells within the total population of GFP+ cells.

Article Snippet: For retrograde labeling of ADn cells: C57BL6j mice were injected with ∼400 nl of rAAV (RetroAAV-hSyn-EGFP, Addgene: 50465) at the following coordinates: −4.5 AP, 2.75 ML, 3.8 dorsoventral (DV) from brain surface.

Techniques: Injection, Labeling, Expressing